What is forward pipetting technique?

Forward pipetting is a standard technique used to dispense aqueous solutions such as buffers, diluted acids, or alkalis. This Technique works on the air displacement method and is commonly used when pipetting and mixing a sample into another liquid.

1. To aspirate the sample in the tip, press the plunger to the first stop and immerse the micropipette tip vertically in the sample.
2. Slowly release the plunger while the tip is immersed. The sample will be aspirated into the micropipette tip.
3. To dispense the sample, place the tip on the inner wall of the receiving vessel at a steep angle.
4. Slowly press the plunger again to the first stop to dispense the sample into the receiving vessel.
5. To empty the tip completely, press the plunger to the second stop.
6. Wipe the tip on the inner wall while taking the tip out to the receiving vessel.